Deoxynivalenol-3-Glucoside Content Is Highly Associated with Deoxynivalenol Levels in Two-Row Barley Genotypes of Importance to Canadian Barley Breeding Programs

Deoxynivalenol-3-Glucoside Content Is Highly Associated with Deoxynivalenol Levels in Two-Row Barley Genotypes of Importance to Canadian Barley Breeding Programs

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Barley (Hordeum vulgare L.) is a multipurpose crop that can be harvested as grain or cut prior to maturity for use as forage.Fusarium head blight (FHB) is a devastating disease of barley that reduces quality of grain.

FHB can also result in the accumulation of mycotoxins such as deoxynivalenol (DON).Breeding FHB resistant varieties has been a long-term goal of many barley-producing countries, including Canada.While the genetic basis of DON detoxification via production of less-phytotoxic conjugates such as DON-3-glucoside (DON3G) is well documented in barley, little information exists in reference to varietal response.

Over two years, 16 spring, two-row barley genotypes, Liebherr CUEL2831 55cm Fridge Freezer – SILVER 265 litres A++ of importance to western Canadian barley breeding programs, were grown as short-rows and inoculated following spike emergence with a Fusarium graminearum conidia suspension.Half of the plots were harvested at soft GROWTH dough stage and then dissected into rachis and grain components, whereas the remainder was harvested at maturity.Multiple Fusarium-mycotoxins were assayed using liquid chromatography-mass spectrometry.

Mycotoxin content was elevated at the earlier harvest point, especially in the rachis tissue.DON3G constituted a significant percentage (26%) of total trichothecene content and thus its co-occurrence with DON should be considered by barley industries.DON3G was highly correlated with DON and 3-acetyl-deoxynivalenol (3ADON).

The ratio of D3G/DON exhibited consistency across genotypes, however more-resistant genotypes were characterized by a higher ratio at the soft-dough stage followed by a decrease at maturity.Plant breeding practices that use DON content as a biomarker for resistance would likely result in the development of barley cultivars with lower total DON-like compounds.